Abdullah Ayed Kanee
Tendaha Secondary School, Khamis Mushait, Asir, SAUDI ARABIA
Lipid rafts play an integral role in cell adhesion. This project investigates the use of Methyl Beta Cyclodextrin to disrupt lipid rafts in chicken embryonic retina cells and the subsequent effect on cell aggregation.<br><br>A Cell Aggregation Assay was used to experiment with different concentrations of Methyl Beta Cyclodextrin . A Cell Viability Assay was used to determine whether the cells were alive after the administration of the 8mM concentration.<br><br>It was found that Methyl Beta Cyclodextrin has an inhibitory effect on cell adhesion, as hypothesized, and that this effect is concentration dependent. <br><br>This paper also discusses cell response to different concentrations of Methyl Beta Cyclodextrin which may prove relevant to future work involving Methyl Beta Cyclodextrin and chicken embryonic cells. The study of retinal stem cells for possible therapies for macular degeneration, retinal degeneration, glaucoma or optic nerve trauma holds great promise in the future.