Anas Mohamed Al Fadda
Riyadh Schools, Riyadh, SAUDI ARABIA
Genome annotation has a huge impact on molecular biology. Therefore, making an
annotated database using Rhodococcus aethreivorans bacteria will allow researchers to
have databanks that they can base upon their new research. Rhodococcus aethreivorans
bacteria has not been annotated before and there is almost no coding in bacterial
molecular biology. This database identifies certain elements called Transpose elements,
that encode important proteins. In order to create this database, inserting the pRA3 script
was the first step. The second step is marking the open reading frames which label genes
in this plasmid. The third step is using BLAST(Basic Local Alignment search tool) search
tool, which is the main tool that is used to identify genes. The fourth step is editing the
gene to differentiate between normal genes and transpose elements. The fifth step is
navigating the start of the sequence to know where the protein actually starts in order to
make the data base more accurate. Also, this annotation of this single plasmid took a
month and a half to be completed. Other genomes take up to 6-12 months. Thus, this will
save scientists a lot of time. In order to further develop this study, the annotation of other
bacteria such as Streptococcus pneumonia, Helicobacter pylori, Staphylococcus aureus,
Streptococcus mutans will be completed. These transpose elements encode important
proteins such as: Replication Protein. Drug companies could focus on these points in
order to make the drug more efficient and concentrated.