Moshira Ashraf Abd El Meguid, Mariam Ashraf Abd El Meguid
Dar El Tarbiah American School, Zamalek, Cairo, Egypt
Human race has been suffering along the ages several highly lethal diseases .By tracing the source of these diseases it was found that one of the main source of these diseases were mosquitoes causing several epidemics .Such diseases are like malaria,West Nile fever, dengue fever,….etc.
Several ways were used to eradicate and control mosquitoes .The development of synthetic pesticides since 1940 coupled with the improvement in chemical application technology, dramatically increased the potential for insect pest control. Pests that had been naturally controlled by predators and parasites began to cause significant damages and became resistant to chemical pesticides. Moreover, chemical pesticides have become costly and imposed great hazards to the environment and all living organisms.
An alternative tool for effective control of pests is the use of Bacillus thuringiensis. Using Bacillus thuringiensis directly on the mosquitoe`s swamp is not efficient due to ultra violet rays which break down the Bt gene toxin.
So we isolated cry11Aa from Bt and cloned it in TA cloning vector and sub cloned in pet30a as expression vector .The purified protein gave a very good mortality against mosquito larvae concentration 224 ng/ml. this biopesticide is environmentally friendly and not harmful for the ecosystem and it does not sediment and is not affected by the ultra violet radiations.