AbdulElah Marwan AlGosaibi, Faisal Alabdulkareem,
Riyadh Schools, RIYADH, CENTRAL, SAUDI ARABIA
Lead is an environmental contaminant that is used significantly in modern industry. The purpose of this project is to study the effect of Lead toxicity on experimental Wister rats which are exposed to 2% Lead acetate in drinking water. The applicability of obtained results for human blood will be examined in the future advancement of this study.
In this project, 32 Wister rats were divided into 4 groups of 8 rats. Group 1 was used as control and was given tap water. Group 2,3 and 4 were treated with 2% Lead acetate in their drinking water for 1, 2 and 3 months respectively.
The following parameters were monitored:
Alanine aminotransferase (ALT), Aspire aminotransferase (AST), alkaline phosphate (ALP). In addition, Hemoglobin absorption spectrum and dielectric measurements were also monitored.
Results showed that rats exposed to Lead for longer period of times experience significant decrease in plasma total protein and significant increase in other parameters as compared to control. This pertains to toxic lever damage and correlate to the development of fibrosis.
In addition, results showed that the Hemoglobin spectra displayed well-known bands at 220-280-340-410-540. This band corresponds to aliphatic amino acids, aromatic amino acids, aromatic amino acids and other acids in liver. This indicates partial loss of Hemoglobin molecule stability. Moreover, free radicals produced in the presence of heavy metals could lead to anemia.