Shikah Duwihi A Alshuwaish
Altarbia Alislamia Schools, Riyadh, SAUDI ARABIA
Magnetic nano-particles have been undergoing studies because of their unique properties. Although functionalization of such particles has been used to substantiate their use in novel technologies like pathogen detection and drug delivery systems, a quantitative study of the capture rate has never been undertaken. In order to develop these technologies, it is essential to quantify the number of entities that have been captured by the magnetic particles. The objective of this project is to determine how different variables affect the capture rate of magnetic nano-particles for their use in biomedical applications. Two experiments were conducted to prove the possibility of attaching biological or non-biological entities to magnetic particles. First, E.coli was used with the amount of bacterial fluid and E.coli antibody as variables after being incubated for 16 hours at 37 degrees and then checked under the microscope to quantify the capture rate of the magnetic beads. Second, fluorescent filled polymers that were diluted 1=100 microliters were attached to the magnetic beads using streptavidin and biotin bond. It was noticed that there was an increase in the capture rate with an increase in the variables. The capture rate was successfully quantified and a direct relationship between the variables and the capture rate was determined. This study is a leap towards developing novel technologies that will revolutionize today`s biomedical applications by providing numbers that can be used in modeling the sensitivity of applications such as Lab on Chip Devices and drug delivery systems.